COURSE OVERVIEW
Who is this course for?
This course is designed for the more experienced chromatographer with a good understanding of GC. We would highly recommend attendees have already completed our Operating and Understanding GC course, although this is not compulsory.
Previous Knowledge
Participants should have a good knowledge of chromatography and experience as GC users. Some experience in method development is also recommended. A good grounding in chemistry will be beneficial.
What you will learn
- Setting method development objectives
- Sample preparation
- Inlet and flow rate parameters
- Choosing a column and temperature program
- Optimisation strategies
COURSE OUTLINE
Establishing Method Development Objectives
- Defining Analytical Goals
- Literature Search and Background Research
- Understanding What is Known vs. What Needs to be Explored
- Introduction to Quality by Design (QbD)
- Identifying Critical Parameters and Robustness
Sample Preparation Considerations
- Sample Clean-Up Strategies
- Extraction Techniques
- Selecting Appropriate Solvents
Inlet, and Flow Rate Optimization
- Effect of Split Ratio on Peak Shape and Quantification
- Investigating Oven Initial Temperature and Ramp Rates
- Conversion of Split Methods to Splitless Methods
- Optimizing Purge-On Time
- Carrier Gas Selection and Flow Rate Optimization (van Deemter & Golay Theory)
Column Selection and Temperature Programming
- Selecting the Right Stationary Phase
- Influence of Column Geometry on Separation
- Solute-Stationary Phase Interactions
- Isothermal vs. Gradient Temperature Programming
- Theory and Practical Development of Temperature Gradients
Optimization Strategies in GC Method Development
- Measuring and Optimizing:
- Capacity Factor
- Efficiency
- Resolution
- Selectivity
- Resolution Equation and Its Application
- Case Study: Developing an Effective GC Method
Finalizing and Validating the Method
- Developing a GC Method for a Complex Mixture
- Systematic Instrument Setup and Validation
Data Processing and Interpretation
- Peak Integration and Data Analysis
- Calibration Methods and Accuracy
- System Suitability Testing
Q&A and Closing Discussion
- Summary of Key Concepts
- Open Discussion on Challenges Faced in GC Method Development
- Resources for Further Learning